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| Id: | 18313
| | Author: | Kaplan, Gilla; Nusrat, Asma; Sarno, Euzenir N; Job, C. K; McElrath, Julie; Porto, Jarbas A; Nathan, Carl F; Cohn, Zanvil A
| | Title: | Cellular response to the intradermal injection of recombinant human gamma-interferon in lepromatous leprosy patients
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| | Source: | s.l; s.n; Aug. 1987. 9 p. ilus, tab.
| | Abstract: | The local response to single intradermal injection of 10 ug recombinat gamma-interferon (rIFNgamma) has been studied in 17 patients with lepromatous leprosy. Of these, 2 patients additionally received two intradermal injections of 10 ug rIFNgamma at received another site. The results were compared with those of 3 patients who received three injections of the same dose at a single site in an earlier study. One to 7 days after lymphokine administration 4-mm pinch biopsies were obtained and axamined for cellular alterations in the dermis and epidermis. This allowed a kinetic analysis of mononuclear cell infiltration, keratinocyte proliferation and differentiation and Langerhans cell redistribution. At 24 hours, the migration of large numbers of helper T cells and monocytes was already prominent and associated with induration. Mononuclear cell eccumulation peaked at 72 hours but then persisted for 5-7 days. Only smal numbers (one-third or less of toal T cells) of suppressor/cytotoxic T cells were present at any time, and granulocytes were absent. Two daily injections of rIFNgamma led to a more intense accumulation of cells. Ten ug of rIFNgamma resulted in enhanced keratinocyte proliferation, Ia expression, and thickening of the epidermis. At 24-48 hours major histocompatibility Class II (Ia) antigen was first noted on the dividing cells of the basal layer. By 72-96 hours the entire epidemir exhibited strong expression of Ia antigen on cell surfaces. Repeated doses of lymphokine accentuated these changes and resulted in a more prompt keratinization and sloughing of this layer. Whereas a single dose of rIFNgamma resulted in the upward movement of T6+ Langerhans cells (LCs) in the epidermis, two injections led to a 50% reduction in their numbers and three doses were associated with an almost total loss of detectable T6+ LCs from the epidermis. These are probably aloughed along with keratinocytes. In contrast to the situation with a delayed immune response in the skin (purified protein derivative), no LCs accumulated in the dermis in association with helper T cells (AU).
| | Descriptors: | HANSENIASE VIRCHOWIANA/genet
HANSENIASE VIRCHOWIANA/imunol
HANSENIASE VIRCHOWIANA/microbiol
HANSENIASE VIRCHOWIANA/fisiopatol
INJECOES INTRADERMICAS/métodos
INJECOES INTRADERMICAS/util
CERATINOCITOS/citol
CERATINOCITOS/imunol
| | Limits: | ESTUDO COMPARATIVO
| | Location: | BR191.1; 09292/s |
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